The 100 bp dna ladder is a unique combination of a. The 500 and 1,500 base pair bands have increased intensity to. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. A unique combination of pcr products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 500 bp fragment and the bp fragment are present at increased intensity to allow easy identification. The 500 bp band contains more dna to provide internal orientation. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. The 500 bp dna ladder consists of 17 bluntended fragments between 500 and 8500 bp in multiples of 500 bp.
The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 100 bp dna ladder is suitable for sizing doublestranded dna fragments from 1002000 bp. The product is formatted as readytoload molecular weight marker containing bromophenol blue as a tracking dye. Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna ranging from 10 bp. It includes three electrophoresis tracking dyes xylene cyanol, bromophenol blue and orange g, allowing the process of the dna through the gel to be visualized.
Readytoload 100 bp plus dna ladder ranging from 100 to. This ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at. Buy online stepup 1kb dna ladder 100 ug 612652071001730 at best price in india on. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. The 50 bp dna ladder is prepared to ensure quality and batchtobatch consistency. Fisher bioreagents routine dna ladder 100 bp low scale ladder life sciences. The dna ladder consists of proprietary plasmids which are. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most poplar markers used for dna fragment size determination. The dna includes fragments ranging from 1001,500 base pairs. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an. This ladder is ideal for quick sizing of pcr products. Generuler 100 bp plus dna ladder 250 5 x 50 g for 500 applications, 0. The 100 bp plus dna ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp.
All bands except the 800bp band are of equal intensity. Dna step ladders have defined sizes with exact incremental steps between bands. The 60 and 100bp bands are invisible to serve as reference points. Promega 100bp dna step ladder molecular weight marker for. Straightforward procedure for laboratory production of dna ladder. The 500bp fragment is present at increased intensity for easy identification. Accuband 100bp dna ladder ii, dm2200, 500ulvial, 200 loads. Source pcr products and doublestranded dna digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mm trishcl ph 8. The 300bp band is 3 times more intense than all other bands.
The 100 bp dna ladder is a unique combination of a number. The dna ladder consists of double strand dna fragments ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. Thermo scientific generuler 100 bp plus dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose and polyacrylamide gels. The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. The ladder is a mixture of chromatographypurified individual dna fragments. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. The key to accurate band analysis is to use the correct dna ladder for your particular application.
Validity the present general terms of sale and delivery apply to all business of vwr international gmbh, i. The 100 bp dna ladder, containing 12 linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 100 bp to 3,000 bp. The approximate mass of dna in each band is provided 0. The 500 bp band with higher concentration is distinguished easily from others. Every readytouse ladder is supplied with the nucleasefree loading dye solution, which ensures optimal migration and quantification of your dna probes.
Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Buy stepup 1kb dna ladder 100 ug 612652071001730 in india. The 500 bp and 1,500 bp bands contain more dna to provide internal orientation. The 500 bp band with higher concentration is distinguished easily from. Generuler 100 bp plus dna ladder thermo fisher scientific. A highintensity 500bp band stands out at the lowest segment of the ladder 1kb. Promega 100bp dna step ladder molecular weight marker for electrophoresis 100bp dna step ladder 100 to 4,000. Fisher bioreagents routine dna ladder 100 bp low scale.
Dna ladders have defined sizes, and are not intended for use in quantitative analysis. The 100 bp dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield 12 fragments, suitable for use as molecular weight standards for electrophoresis. The ladder is generated from pcr and restriction enzyme digestion of double stranded dna. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. The dna ladder consists of double strand dna fragments ranging in sizes. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. Generuler 100 bp dna ladder, readytouse 100 to bp is recommended for sizing and approximate quantification of a wide range of doublestranded dna fragments on agarose or polyacrylamide gels. Dna ladder is commonly used to determine the size of dna fragments by electrophoresis in routine molecular biology laboratories. This marker is ideal for the size determination of pcr products. The dna fragments may be stained with ethidium bromide. The digested dna includes fragments ranging from 100 1500 base pairs.
This ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. The 100 bp dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. The ladder is prepared from a plasmid containing repeats of a 100 bp dna fragment. Sixteen dna fragments ranging from 50bp to 800bp in 50bp increments plus an 1,800bp backbone.
In this study, we report a new procedure to prepare a dna ladder that. Trackit 100 bp dna ladder is ideal for separation on 12% agarose gels. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel loading dye, purple 6x, no sds. The dna includes fragments ranging from 1003,000 base pairs. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. The ladders are not intended for use in quantitative analysis. The quickload purple 100 bp dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Dna marker 100 bp plus is designed for use as qualitative dna reference marker availibility of sample size may be limited. The product is formatted as readytoload molecular weight marker containing bromophenol.
The 100 plus dna marker was designed for precise qualitative and approximately quantification of dna mass. Quickload purple 100 bp dna ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This product is available in quickload purple format enjoy the convenience of a readytoload format. Filter the results on this page to find the product best suited for your application. The 2000 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation.
Two internal features facilitate band identification. The 10 bp dna ladder consists of nine 10bp repeats and is suitable for sizing doublestranded dna fragments from 20 bp to 140 bp. The 100 bp dna ladder is suitable for sizing doublestranded dna fragments from 100 2000 bp. The 100 bp ladder marker bp from geneon is designed for use as molecular weight standard for agarose gel electrophoresis. Quickload purple 100 bp dna ladder is stable for at least 6 months at 25c. Each band contains 50ng of dna, with the 500bp band intensity increased 150ng to serve as a reference. The readytouse 100 bp plus dna ladder comes premixed with loading buffer dye and ranges between 3000 to 100 bp. Sixteen dna fragments ranging from 50bp to 800bp in 50bp increments plus an 1,800bp backbone fragment. The dna includes fragments ranging from 100 3,000 base pairs. The digested dna includes fragments ranging from 100 bp to 1,517 bp.
The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm. Thermo scientific generuler 100 bp plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels. It allows sizing and concentration estimate of dna fragments on agarose gels generated by pcr or restriction digest. Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. Agarose gel analysis must show that bands between 100 1500 bp are distinguishable after ethidium bromide staining. Trackit 100 bp dna ladder thermo fisher scientific. The 100bp dna step ladder has 40 bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information. The 100 bp dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to bp.